mini protean tetracell system (Bio-Rad)

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Bio-Rad mini protean tetracell system
Mini Protean Tetracell System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 3013 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mini+protean+tetracell/Mini-PROTEAN+Tetra+Cell/pm41898438-227-8-11
Average 97 stars, based on 3013 article reviews

mini protean tetracell system - by Bioz Stars, 2026-09

97/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Membraneless protocell confined by a heat flow
Article Snippet: An unstained protein ladder (26630, Thermo Scientif ic) was used as reference. .. Gel electrophoresis was conducted in a Mini-PROTEAN tetracell (1658004, Bio-Rad) or midi CriterionTM cell (1656001, Bio-Rad) with 1x Tris-Glycine-SDS buf fer (3060.3, Roth) at 200 V. Gel images were acquired with a ChemiDoc MP Imaging System (Bio-Rad) with rapid auto-exposure. ..

Imaging:

Electrophoresis:

Article Title: Improvement of techno-functional properties of acidic subunit from amaranth 11S globulin modified by bioactive peptide insertions
Article Snippet: .. Electrophoresis was performed using a mini-Protean Tetracell (Bio-Rad, Hercules, CA, USA) at constant voltage (100 V) for 90 min., as protein molecular weight marker was used, PageRulerTM unstained broad range protein ladder ThermofisherTM, and Blu10 Plus (BLUltra) prestained protein ladder for the western blot. .. Finally, a refolding protocol was applied to eliminate urea and allow refolding of each recombinant protein through the dialysis process as follows: first, 2 mL at 0.5 and 1 mg/mL for each sample of protein was dialyzed against 50 mL of refolding buffer (4 M urea, 0.2 M NaCl, 20 mM phosphates, pH 7.5) for 1.5 h in an analog SRT9D tube roller (Bibby Scientific, Stone, Staffordshire, UK) at 4 C. This process was repeated to diminish the urea concentration to 3, 1.5, and 0.5 M. Finally, three cycles of dialysis were applied against 50 mL of distilled water and freeze-dried.

Molecular Weight:

Article Title: Stimulus-responsive assembly of nonviral nucleocapsids.
Article Snippet: Briefly, the sample was incubated with NuPAGE LDS Sample Buffer (4X) (Thermo Fisher Scientific, Waltham, MA, USA) at 70 °C for 10min. .. The resulting mixture and a protein molecular weight marker, PageRuler prestained protein ladder (Thermo Fisher Scientific, Waltham, MA, USA), were separated on a 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad Laboratories Inc., Hercules, CA, USA) at 200V for 30min using a Mini-PROTEAN TetraCell and PowerPacUniversal Power Supply (Bio-Rad Laboratories Inc., Hercules, CA, USA). .. The gel was stainedwith a solution containing 0.1% Coomassie Brilliant Blue R-250, 40% MeOH, and 10% acetic acid with gentle agitation for 1 h. After destaining in 40% MeOH and 10% acetic acid, the gel image was recorded with a ChemiDoc MP Imaging System (Bio-Rad Laboratories Inc., Hercules, CA, USA).

Marker:

Western Blot:

SDS Page:

Article Title: The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.
Article Snippet: Flow cytometry acquisitions were performed on a BD LSR II cytometer (BD Biosciences), and data were analyzed with Cytobank (https://www.cytobank.org).71 .. Samples were diluted with Laemmli buffer (Biorad) and NuPAGE sample reducing agent (Invitrogen) and heated at 95 C for 5 min. Proteins were then separated by SDS-PAGE using a mini-PROTEAN Tetracell (Biorad), and transferred to nitrocellulose membrane with a mini-TransBlot Cell (Biorad). ..

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